全文获取类型
收费全文 | 7665篇 |
免费 | 842篇 |
国内免费 | 3篇 |
出版年
2023年 | 47篇 |
2022年 | 51篇 |
2021年 | 245篇 |
2020年 | 134篇 |
2019年 | 177篇 |
2018年 | 205篇 |
2017年 | 193篇 |
2016年 | 339篇 |
2015年 | 525篇 |
2014年 | 522篇 |
2013年 | 537篇 |
2012年 | 694篇 |
2011年 | 717篇 |
2010年 | 459篇 |
2009年 | 357篇 |
2008年 | 503篇 |
2007年 | 505篇 |
2006年 | 381篇 |
2005年 | 378篇 |
2004年 | 385篇 |
2003年 | 329篇 |
2002年 | 301篇 |
2001年 | 56篇 |
2000年 | 41篇 |
1999年 | 48篇 |
1998年 | 63篇 |
1997年 | 28篇 |
1996年 | 31篇 |
1995年 | 25篇 |
1994年 | 23篇 |
1993年 | 16篇 |
1992年 | 16篇 |
1991年 | 18篇 |
1990年 | 13篇 |
1989年 | 8篇 |
1988年 | 3篇 |
1987年 | 5篇 |
1986年 | 8篇 |
1985年 | 11篇 |
1984年 | 16篇 |
1983年 | 8篇 |
1982年 | 9篇 |
1981年 | 21篇 |
1980年 | 13篇 |
1979年 | 8篇 |
1978年 | 10篇 |
1977年 | 6篇 |
1976年 | 5篇 |
1975年 | 3篇 |
1968年 | 3篇 |
排序方式: 共有8510条查询结果,搜索用时 187 毫秒
101.
Jozef Dingemans Rebecca E. Al‐Feghali Gee W. Lau Karin Sauer 《Molecular microbiology》2019,111(5):1211-1228
The hybrid sensor SagS plays a central role in the formation of Pseudomonas aeruginosa biofilms, by enabling the switch from the planktonic to the biofilm mode of growth and by facilitating the transition of biofilm cells to a highly tolerant state. In this study, we examined the importance of the SagS key amino acid residues associated with biofilm formation (L154) and antibiotic tolerance (D105) in P. aeruginosa virulence. Recombinant P. aeruginosa ΔsagS and ΔsagS chromosomally expressing wild‐type sagS, or its two variants D105A and L154A, were tested for their potential to form biofilms and cause virulence in plants and mouse models of acute and chronic pneumonia. Although mutation of sagS did not alter P. aeruginosa virulence during acute infections, a significant difference in pathogenicity of sagS mutants was observed during chronic infections, with the L154A variant showing reduced bacterial loads in the chronic pneumonia model, while interference with the D105 residue enhanced the susceptibility of P. aeruginosa biofilms during tobramycin treatment. Our findings suggest that interference with the biofilm or tolerance regulatory circuits of SagS affects P. aeruginosa pathogenicity in chronic but not acute infections, and reveal SagS to be a promising new target to treat P. aeruginosa biofilm infections. 相似文献
102.
103.
Transgenic Research - 相似文献
104.
Vicuna Requesens Deborah Gonzalez Romero Maria Elena Devaiah Shivakumar P. Chang Yeun-Kyung Flory Ashley Streatfield Stephen Ring Rebecca Phillips Cassie Hood Nathan C. Marbaniang Cyrus Dean Howard John A. Hood Elizabeth E. 《Transgenic research》2019,28(5-6):537-547
Transgenic Research - Expression of recombinant proteins in plants is a technology for producing vaccines, pharmaceuticals and industrial enzymes. For the past several years, we have produced... 相似文献
105.
Rebecca A. Fletcher Rachel K. Brooks Vasiliy T. Lakoba Gourav Sharma Ariel R. Heminger Christopher C. Dickinson Jacob N. Barney 《Global Change Biology》2019,25(11):3694-3705
Despite our growing understanding of the impacts of invasive plants on ecosystem structure and function, important gaps remain, including whether native and exotic species respond differently to plant invasion. This would elucidate basic ecological interactions and inform management. We performed a meta‐analytic review of the effects of invasive plants on native and exotic resident animals. We found that invasive plants reduced the abundance of native, but not exotic, animals. This varied by animal phyla, with invasive plants reducing the abundance of native annelids and chordates, but not mollusks or arthropods. We found dissimilar impacts among “wet” and “dry” ecosystems, but not among animal trophic levels. Additionally, the impact of invasive plants increased over time, but this did not vary with animal nativity. Our review found that no studies considered resident nativity differences, and most did not identify animals to species. We call for more rigorous studies of invaded community impacts across taxa, and most importantly, explicit consideration of resident biogeographic origin. We provide an important first insight into how native and exotic species respond differently to invasion, the consequences of which may facilitate cascading trophic disruptions further exacerbating global change consequences to ecosystem structure and function. 相似文献
106.
107.
Tania Chavarria‐Pizarro Juan Pablo Gomez Judit Ungvari‐Martin Rachael Bay Michael M. Miyamoto Rebecca Kimball 《Ecology and evolution》2019,9(24):13902-13918
Despite the enormous advances in genetics, links between phenotypes and genotypes have been made for only a few nonmodel organisms. However, such links can be essential to understand mechanisms of ecological speciation. The Costa Rican endemic Mangrove Warbler subspecies provides an excellent subject to study differentiation with gene flow, as it is distributed along a strong precipitation gradient on the Pacific coast with no strong geographic barriers to isolate populations. Mangrove Warbler populations could be subject to divergent selection driven by precipitation, which influences soil salinity levels, which in turn influences forest structure and food resources. We used single nucleotide polymorphisms (SNPs) and morphological traits to examine the balance between neutral genetic and phenotypic divergence to determine whether selection has acted on traits and genes with functions related to specific environmental variables. We present evidence showing: (a) associations between environmental variables and SNPs, identifying candidate genes related to bill morphology (BMP) and osmoregulation, (b) absence of population genetic structure in neutrally evolving markers, (c) divergence in bill size across the precipitation gradient, and (d) strong phenotypic differentiation (PST) which largely exceeds neutral genetic differentiation (FST) in bill size. Our results indicate an important role for salinity, forest structure, and resource availability in maintaining phenotypic divergence of Mangrove Warblers through natural selection. Our findings add to the growing body of literature identifying the processes involved in phenotypic differentiation along environmental gradients in the face of gene flow. 相似文献
108.
Rebecca J. Wilson Stephen J. Brooks Phillip B. Fenberg 《Ecology and evolution》2019,9(18):10305-10316
Body size has been shown to decrease with increasing temperature in many species, prompting the suggestion that it is a universal ecological response. However, species with complex life cycles, such as holometabolous insects, may have correspondingly complicated temperature–size responses. Recent research suggests that life history and ecological traits may be important for determining the direction and strength of temperature–size responses. Yet, these factors are rarely included in analyses. Here, we aim to determine whether the size of the bivoltine butterfly, Polyommatus bellargus, and the univoltine butterflies, Plebejus argus and Polyommatus coridon, change in response to temperature and whether these responses differ between the sexes, and for P. bellargus, between generations. Forewing length was measured using digital specimens from the Natural History Museum, London (NHM), from one locality in the UK per species. The data were initially compared to annual and seasonal temperature values, without consideration of life history factors. Sex and generation of the individuals and mean monthly temperatures, which cover the growing period for each species, were then included in analyses. When compared to annual or seasonal temperatures only, size was not related to temperature for P. bellargus and P. argus, but there was a negative relationship between size and temperature for P. coridon. When sex, generation, and monthly temperatures were included, male adult size decreased as temperature increased in the early larval stages, and increased as temperature increased during the late larval stages. Results were similar but less consistent for females, while second generation P. bellargus showed no temperature–size response. In P. coridon, size decreased as temperature increased during the pupal stage. These results highlight the importance of including life history factors, sex, and monthly temperature data when studying temperature–size responses for species with complex life cycles. 相似文献
109.
110.
Alesci Marco Smith Rebecca L. Ayala Santacruz Jorge Damian Camperio Ciani Andrea 《Primates; journal of primatology》2022,63(2):161-171
Primates - Increasing urbanisation is encroaching into natural habitats and sometimes forcing wildlife into urban centres. Whether or not wildlife can thrive in an urban environment is dependent on... 相似文献